JCOGP
Journal of Controversies in Obstetrics & Gynecology and Pediatrics

As of January 2023, our "Journal of Controversies Obstetrics & Gynecology and Pediatrics" has been published under the Medihealth Academy to publish all articles, reviews and case reports on Pediatrics, especially in the field of Obstetrics and Gynecology. In order to facilitate the citation of the articles, to take our place in internationally respected indexes and to reach a wider readership, we will pay attention to the fact that our article language is only English in terms of acceptance.

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Original Article
Morphological analyses of sperms causing severe male infertility with light microscopy, motile sperm organellar morphology examination and electron microscopy tecnics.
Abstract
To evaluate abnormal sperm morphology by three different methods including light microscope, real-time motile sperm organelle morphology assessment at 8050x high magnification and electron microscope and to determine degree of affect on morphologic defects level. Beside the light and electron microscopic techniques, the motile sperm organelle examination can offer information about sperm quality in a simple and quick way and by the IMSI developed through the use of sperm selected by this technique can contribute to the success of in vitro fertilization centers. We believe that sperm selection at high magnifications can increase IVF pregnancy rates in patients with sperm morphology that causes severe male infertility.

Keywords : Male infertility, IMSI, severe sperm morphology

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1. Inhorn MC, Patrizio P. Infertility around the globe: new thinking ongender, reproductive technologies and global movements in the 21stcentury. Hum Reprod Update. 2015;21(4):411-426. doi:10.1093/humupd/dmv016
2. Karaca N, Yilmaz R, Kanten GE, Kervancioglu E, Solakoglu S,Kervancioglu ME. First successful pregnancy in a globozoospermicpatient having homozygous mutation in SPATA16. Fertil Steril.2014;102(1):103-107. doi:10.1016/j.fertnstert.2014.04.002
3. Karaca N, Akpak YK, Oral S, Durmus T, Yilmaz R. A successful healthychildbirth in a case of total globozoospermia with oocyte activation bycalcium ionophore. J Reprod Infertil. 2015;16(2):116-120.
4. van den Hoven L, Hendriks JC, Verbeet JG, Westphal JR, Wetzels AM.Status of sperm morphology assessment: an evaluation of methodologyand clinical value. Fertil Steril. 2015;103(1):53-58. doi:10.1016/j.fertnstert.2014.09.036
5. Kruger TF, Acosta AA, Simmons KF, Swanson RJ, Matta JF,Oehninger S. Predictive value of abnormal sperm morphology in invitro fertilization. Fertil Steril. 1988;49(1):112-117. doi:10.1016/s0015-0282(16)59660-5
6. Bartoov B, Berkovitz A, Eltes F, Kogosowski A, Menezo Y, Barak Y. Real-time fine morphology of motile human sperm cells is associated with IVF-ICSI outcome. J Androl. 2002;23(1):1-8. doi:10.1002/j.1939-4640.2002.tb02595.x
7. World Health Organization. (2010). WHO laboratory manual for theexamination and processing of human semen, 5th ed. World HealthOrganization. https://apps.who.int/iris/handle/10665/44261
8. Lindsay TJ, Vitrikas KR. Evaluation and treatment of infertility[published correction appears in Am Fam Physician. 2015 Sep 15;92(6):437]. Am Fam Physician. 2015;91(5):308-314.
9. Wang C, Swerdloff RS. Limitations of semen analysis as a test ofmale fertility and anticipated needs from newer tests. Fertil Steril.2014;102(6):1502-1507. doi:10.1016/j.fertnstert.2014.10.021
10. Mangoli E, Khalili MA. The Beneficial Role of Intra CytoplasmicMorphologically Selected Sperm Injection (IMSI) in AssistedReproduction. J Reprod Infertil. 2020;21(1):3-10.
11. Kahraman S, Akarsu C, Cengiz G, et al. Fertility of ejaculated andtesticular megalohead spermatozoa with intracytoplasmic sperminjection. Hum Reprod. 1999;14(3):726-730. doi:10.1093/humrep/14.3.726
12. Deveneau NE, Sinno O, Krause M, et al. Impact of sperm morphologyon the likelihood of pregnancy after intrauterine insemination. FertilSteril. 2014;102(6):1584-90.e2. doi:10.1016/j.fertnstert.2014.09.016
13. Kruger TF, Coetzee K. The role of sperm morphology in assistedreproduction. Hum Reprod Update. 1999;5(2):172-178. doi:10.1093/humupd/5.2.172
14. Setti AS, Paes de Almeida Ferreira Braga D, Iaconelli A Jr, Aoki T,Borges E Jr. Twelve years of MSOME and IMSI: a review. Reprod BiomedOnline. 2013;27(4):338-352. doi:10.1016/j.rbmo.2013.06.011
15. Eliasson R. Semen analysis. Environ Health Perspect. 1978;24:81-85.doi:10.1289/ehp.782481
16. Eliasson R. Semen analysis with regard to sperm number, spermmorphology and functional aspects. Asian J Androl. 2010;12(1):26-32.doi:10.1038/aja.2008.58
17. Nistal M, Paniagua R, Herruzo A. Multi-tailed spermatozoa in a casewith asthenospermia and teratospermia. Virchows Arch B Cell Pathol.1977;26(2):111-118. doi:10.1007/BF02889540
18. Sermondade N, Hafhouf E, Dupont C, et al. Successful childbirth afterintracytoplasmic morphologically selected sperm injection withoutassisted oocyte activation in a patient with globozoospermia. HumReprod. 2011;26(11):2944-2949. doi:10.1093/humrep/der258
19. Chelli MH, Albert M, Ray PF, et al. Can intracytoplasmicmorphologically selected sperm injection be used to select normal-sized sperm heads in infertile patients with macrocephalic sperm headsyndrome?. Fertil Steril. 2010;93(4):1347.e1-1347.e13475. doi:10.1016/j.fertnstert.2008.10.059
20. Escalier D. Human spermatozoa with large heads and multiple flagella:a quantitative ultrastructural study of 6 cases. Biol Cell. 1983;48(1):65-74. doi:10.1111/j.1768-322x.1984.tb00203.x
21. Singh G. Ultrastructural features of round-headed human spermatozoa.Int J Fertil. 1992;37(2):99-102.
22. Bendayan M, Caceres L, Saïs E, et al. Human Sperm Morphologyas a Marker of Its Nuclear Quality and Epigenetic Pattern. Cells.2022;11(11):1788. Published 2022 May 30. doi:10.3390/cells11111788
23. Franco JG Jr, Oliveira JBA. MSOME/IMSI versus epigenetic marks:doubts in the past for hopes in the future. JBRA Assisted Reproduction.2022;26(4):567. Published 2022 Nov 9. doi:10.5935/1518-0557.20220054
24. Alvarez Sedó C, Rawe VY, Chemes HE. Acrosomal biogenesis inhuman globozoospermia: immunocytochemical, ultrastructural andproteomic studies. Hum Reprod. 2012;27(7):1912-1921. doi:10.1093/humrep/des126
25. Chemes HE, Brugo S, Zanchetti F, Carrere C, Lavieri JC. Dysplasia ofthe fibrous sheath: an ultrastructural defect of human spermatozoaassociated with sperm immotility and primary sterility. Fertil Steril.1987;48(4):664-669. doi:10.1016/s0015-0282(16)59482-5
26. Chemes HE, Alvarez Sedo C. Tales of the tail and sperm head aches:changing concepts on the prognostic significance of sperm pathologiesaffecting the head, neck and tail. Asian J Androl. 2012;14(1):14-23.doi:10.1038/aja.2011.168
27. Mitchell V, Rives N, Albert M, et al. Outcome of ICSI with ejaculatedspermatozoa in a series of men with distinct ultrastructural flagellarabnormalities. Hum Reprod. 2006;21(8):2065-2074. doi:10.1093/humrep/del130
28. Perotti ME, Gioria M. Fine structure and morphogenesis of “headless”human spermatozoa associated with infertility. Cell Biol Int Rep. 1981;5(2):113.doi:10.1016/0309-1651(81)90018-7
29. Chemes HE, Puigdomenech ET, Carizza C, Olmedo SB, Zanchetti F,Hermes R. Acephalic spermatozoa and abnormal development of thehead-neck attachment: a human syndrome of genetic origin. HumReprod. 1999;14(7):1811-1818. doi:10.1093/humrep/14.7.1811
30. Mangoli, E, Khalili, MA, Talebi, AR, et al. IMSI procedure improvesclinical outcomes and embryo morphokinetics in patients withdifferent aetiologies of male infertility. Andrologia. 2019; 51:e13340.
31. Lukaszuk K, Jakiel G, Woclawek Potocka I, et al. IMSI-Guidelinesfor Sperm Quality Assessment. Diagnostics (Basel). 2022;12(1):192.Published 2022 Jan 13. doi:10.3390/diagnostics12010192
Article available in :
Volume 1, Issue 3, 2023
Page : 54-61
https://doi.org/10.51271/JCOGP-0013
Article Information
Received : 13 Haz 2023
Accepted : 13 Tem 2023
Published : 30 Tem 2023
Corresponding Author :

Ferhat Cengiz: Medistate Hospital, Department of Invitro Fertilization Laboratory Istanbul, Turkey

[email protected]
Citation : Cengiz F. Morphological analyses of sperms causing severe male infertility with light microscopy, motile sperm organellar morphology examination and electron microscopy technics. J Controv Obstetr Gynecol Ped. 2023;1(3):54-61.

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